Practical Course on Targeted Proteomics
17-22 November 2024
Overview
Targeted proteomics technologies, and specially data-independent acquisition techniques, have revolutionized the landscape of proteomic research in the last decade offering researchers unprecedented precision and depth in proteome analysis. The Practical Course on Targeted Proteomics aims to fill in the gap between theory and the actual implementation of the targeted proteomics workflow, so that by the end of the week the students have the necessary know-how to implement the targeted proteomics workflows in their own research laboratories, and gain exposure to emerging applications in the field. The course will offer a daily keynote talk by a high-profile speaker introducing the topic of the day with examples of his/her own research, followed by practical demonstrations, and hands-on tutorials and exercises that will cover the complete workflow for experimental design and data analysis. Moreover, these sessions will be followed by poster sessions and networking events to introduce informal discussions around each participant's particular scientific interests.
Download the Poster:
Scientific Organisers
Speakers
Programme
Sunday 17th Nov 2024 - Welcome
|
19:30-22:00 |
Welcome Dinner |
Monday 18th Nov 2024 - Topic: Introduction to Targeted Proteomics
|
10:00-11.00 |
Biological significance of targeted proteomics Michael MacCoss, Opening Keynote |
|
11:00-11.30 |
- coffee break -
|
|
11.30-12.15 |
Fundamental concepts on targeted proteomics Eva Borràs, Lecture |
|
12:15-13:00 |
Introduction to the Skyline software Brendan MacLean, Lecture |
|
13:00-14:00 |
- lunch -
|
|
14:00-15:30 |
Tutorial 1: Targeted proteomics method development, data analysis and protein quantification using single point calibration Cristina Chiva and Guadalupe Espadas, Practical Session |
|
15:30-16:00 |
- coffee break -
|
|
16:00-17:30 |
Tutorial 1: Targeted proteomics method development, data analysis and protein quantification using single point calibration Cristina Chiva and Guadalupe Espadas, Practical Session |
|
17:30-18:30 |
Apero (Networking Session) |
Tuesday 19th Nov 2024 - Quantitative targeted proteomics methods and applications
|
9:00-10.00 |
Skyline Jam Session Brendan MacLean, Practical Session |
|
10:00-11:00 |
Advanced targeted proteomics methods Cristina Jacob, Keynote |
|
11:00-11:30 |
- coffee break -
|
|
11.30-12.15 |
Quantitative targeted proteomics Cristina Chiva, Lecture |
|
12:15-13:00 |
Targeted proteomics applications in palaeoproteomics Claire Koenig, Lecture |
|
13:00-14:00 |
- lunch -
|
|
14:00-15:30 |
Tutorial 2: Developing an advanced PRM acquisition method - SureQuant Cristina Chiva and Guadalupe Espadas, Practical Session |
|
15:30-16:00 |
- coffee break -
|
|
16:00-17:30 |
Tutorial 3: Absolute quantification using external and internal calibration curves Cristina Chiva and Guadalupe Espadas, Practical Session |
|
17:30-18:30 |
Apero + Poster Session (Networking Session) |
Wednesday 20th Nov 2024 - High-throughput targeted proteomics I
|
9:00-10.00 |
Skyline Jam Session Brendan MacLean, Practical Session |
|
10:00-11:00 |
Introduction to data-independent acquisition datasets Eduard Sabidó, Keynote |
|
11:00-11:30 |
- coffee break -
|
|
11.30-12.15 |
Library prediction for data-independent acquisition datasets Mathias Wilhelm, Lecture |
|
12:15-13:00 |
Peptide centric approaches in data-independent acquisition Michael MacCoss, Lecture |
|
13:00-14:00 |
- lunch -
|
|
14:00-15:30 |
Tutorial 4: Peptide-centric analysis of DIA datasets Eva Borras & Eduard Sabido, Practical Session |
|
15:30-16:00 |
- coffee break -
|
|
16:00-17:30 |
Tutorial 4: Peptide-centric analysis of DIA datasets Eva Borras & Eduard Sabido, Practical Session |
|
17:30-18:30 |
Apero + Poster Session (Networking Session) |
Thursday 21st Nov 2024 - High-throughput targeted proteomics II
|
9:00-10.00 |
Skyline Session Brendan MacLean, Practical Session |
|
10:00-11:00 |
Spectrum centric approaches in data-independent acquisition Alexey Nesvizhkii, Keynote |
|
11:00-11:30 |
- coffee break -
|
|
11.30-12.15 |
Fast label-free quantification using data-independent acquisition Jesper V Olsen, Lecture |
|
12:15-13:00 |
DIA proteomics: achieving sensitivity and quantitative accuracy Vadim Demichev, Lecture |
|
13:00-14:00 |
- lunch -
|
|
14:00-16:30 |
Tutorial 5: Spectrum-centric analysis of DIA datasets Eva Borras & Eduard Sabido, Practical Session |
|
16:30-22:00 |
Social Event + Dinner (Networking Session) |
Friday 22nd Nov 2024 - High-throughput targeted proteomics III
|
9:00-10.00 |
Skyline Session Brendan MacLean, Practical Session |
|
10:00-11:00 |
Unifying the analysis of bottom-up proteomics data with Chimerys Martin Frejno, Keynote |
|
11:00-11:30 |
- coffee break -
|
|
11.30-12.15 |
Experimental design, statistical analysis and data reproducibility of targeted proteomics data Anthony Wu, Lecture |
|
12:15-13:00 |
Case study and walk through the MSstats platform Anthony Wu, Keynote |
|
13:00-14:00 |
- lunch -
|
|
14:00-14:45 |
Simultaneous targeted and discovery-driven clinical proteotyping using hybrid-PRM/DIA Sandra Goetze, Lecture |
|
14:45-15:45 |
Single-cell proteomics data-independent acquisition methods Claudia Ctortecka, Closing Keynote |
|
15:45-16:30 |
Farewell Drinks |
Call for abstracts
Abstract submission is mandatory to attend the course.
Abstract guidelines
- Abstracts (in English) must be submitted within the online application no later than the 10th September 2024. Submission of an abstract does not ensure acceptance of the course. Abstracts should include a title, authors, authors’ institution addresses, and body.
- Bibliographic references should only be used if quoted in the abstract body. The body of the abstract should not exceed 500 words in English.
- Without the submitted abstract, the application will not be considered for further selection process. Poster specifications.
- The course includes several poster sessions in which participants will explain their research projects. Each participant will be provided with an A0 poster board to display the poster.
- The poster board surface area is approx. 90 cm (about 2.95 ft) wide and 120 cm (about 3.94 ft) high. This is a portrait/vertical format. Materials should be printed on thin poster paper or cardboard, anything heavier will not stay in position. You will be provided with velcro tabs and pins to adhere your poster to the board. All participants need to present their poster.
Registration
REGISTRATION FEES
- Academic 500 EUR (+21% VAT)
- Industry 1000 EUR (+21% VAT)
REGISTRATION DEADLINE
23rd September 2024
ABSTRACT SUBMISSION DEADLINE
23rd September 2024
CHOSEN PARTICIPANTS WILL BE NOTIFIED BY
25th September 2024
PAYMENT DEADLINE
6th October 2024
Registration includes:
- Participation in all course sessions
- Course materials
- Accommodation (in a shared room)
- Lunches, coffee breaks, and some dinners
- Welcome dinner
- Social Activity
Payment
Selected participants will receive an email with a link to do the online payment.
Selection criteria
Submission of an abstract is mandatory. Please also upload a motivation letter via the registration system.
In case more than 25 applicants register for this course, the following criteria will be applied to select the course participants:
- As each applicant will be requested to submit a short abstract summarizing their project and how targeted proteomics might be useful for their research in the future, the selection of participants will be based on their motivation and skills.
- Each applicant will be expected to have a basic prior knowledge of MS-based proteomics.
- Fair distribution of applicants from different countries and scientific institutes.
- Fair gender distribution.
- Fair background distribution: students, postdocs, senior scientists, and industry participants.
Travel grants and registration fee waivers
A limited number of travel grants and registration fee waivers are available for participants.
Applicants do not need to apply separately for these but should indicate on the registration form if they wish to be considered.
Sponsors
Contact and venue
CRG Training and Academic Office
Email: training@crg.eu
Phone: +34933160375
CRG – Centre for Genomic Regulation
Dr. Aiguader, 88
08003 Barcelona, Spain
Accommodation
Accommodation will be arranged and covered by the organizers for both speakers and participants at the same hotel. Participants will be provided with accommodation in double shared rooms with other participants from the course (the organizers will prepare the rooming list based on gender).
Transport
By plane:
By train:
The city's main station is Estació de Sants. This station is serviced by numerous national and international trains, including high-speed trains.
Barcelona has other train stations:
- Estació de França, near Parc de la Ciutadella
- Plaça de Catalunya, in the city centre.
- Clot-Aragó, near Plaça de les Glòries.
- Arc de Triomf, station has a connection with Barcelona Nord bus station.
- Passeig de Gràcia, at the heart of the Modernista route.
Long-distance journeys are best booked in advance but for shorter journeys, including those in Barcelona on the local Rodalies (Catalan) or Cercanías (Spanish) services, you can buy a ticket before boarding the train.
Barcelona is also served by the Ferrocarrils de la Generalitat de Catalunya (FGC). Although they run short and medium-distance inter-city train services, these are integrated into the city's underground network, the Metro, which means you can use the T-10, a combined ticket for 10 journeys on city buses and the underground as well as the FGC trains.
By bus:
The main bus station is Barcelona Nord, which is very near the center, by the Arc de Triomf, although most buses stop at Sants station. There are national and international services and usually, it is not necessary to book in advance. The bus station is linked to the city’s underground system. Look at the map with the different underground lines and bus routes on the TMB website.
Transport from the Barcelona Airport El Prat
You can get to the city from the airport with the Aerobús with stops in Plaça d’Espanya, Urgell, Plaça de la Universitat and Plaça de Catalunya, or the underground line L9. The airport train station is located 200 meters outside the terminal T2 building and the train goes to the Barcelona Sants Estació.
There is also a standard bus and night bus. Click here for more information.
Supported by
CRG Trustees
.png)
Distinction
